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AXL ELISA 试剂盒

This 小鼠 AXL ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 AXL.
Rockland
产品编号 ABIN6720183
Supplier Product No.: koa0377
发货至: 中国

Quick Overview for AXL ELISA 试剂盒 (ABIN6720183)

抗原

See all AXL ELISA试剂盒
AXL (AXL Receptor tyrosine Kinase (AXL))

适用

  • 8
  • 3
  • 2
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0377

    Supplier

    Rockland

    原理

    Mouse AXL Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse AXL. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse AXL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse AXL standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: H20-P443

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all AXL ELISA试剂盒

    AXL (AXL Receptor tyrosine Kinase (AXL))

    别名

    AXL

    背景

    Synonyms: Adhesion related kinase, AI323647, Ark, AXL oncogene, AXL receptor tyrosine kinase, AXL transforming gene, AXL transforming sequence/gene, JTK11, Oncogene AXL, Tyro7, Tyrosine protein kinase receptor UFO, UFO

    Background: Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.

    基因ID

    26362

    NCBI登录号

    NP_033491

    UniProt

    Q00993

    途径

    RTK signaling, Cellular Response to Molecule of Bacterial Origin
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