GNLY ELISA 试剂盒
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Quick Overview for GNLY ELISA 试剂盒 (ABIN6720169)
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See all GNLY ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0756
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Supplier
- Rockland
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原理
- Human Granulysin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: M1-L145 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Granulysin (GNLY) ELISA Kit
GNLY 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 AA 1-145 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 3.12-200 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 234 pg/mL - 15000 pg/mL Cell Culture Supernatant, Plasma, Serum
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Granulysin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Granulysin standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.3%, 4.9%, 6.6%
Inter-Assay Precision (CV%): 7.4%, 5%, 7.3% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- GNLY (Granulysin (GNLY))
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别名
- Granulysin
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背景
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Background: Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.
Gene Full Name: granulysin
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基因ID
- 10578
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NCBI登录号
- NP_001289687
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UniProt
- P22749
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