GNLY ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for GNLY ELISA 试剂盒 (ABIN1889422)
抗原
See all GNLY ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 31.2 pg/mL
-
原理
- Sandwich Human Granulysin ELISA Kit to quantitate Human GNLY in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: M1-L145Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Granulysin (GNLY) ELISA Kit
GNLY 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 3.12-200 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulysin (GNLY) ELISA KitGNLY 适用: 人 Colorimetric Sandwich ELISA 234 pg/mL - 15000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 7.3%, 4.9%, 6.6%
Inter-Assay Precision (CV%): 7.4%, 5%, 7.3% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- GNLY (Granulysin (GNLY))
-
别名
- GNLY
-
背景
-
Background: Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.
Gene Full Name: granulysin
-
基因ID
- 10578
-
UniProt
- P22749
抗原 See all GNLY ELISA试剂盒
-
