CD31 ELISA 试剂盒
Quick Overview for CD31 ELISA 试剂盒 (ABIN6720135)
抗原
See all CD31 (PECAM1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0528
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Supplier
- Rockland
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原理
- Mouse PECAM-1 - CD31 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse PECAM-1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit
PECAM1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 小鼠 AA 18-590 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 人 AA 28-601 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse PECAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse PECAM-1 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: E18-K590
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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别名
- PECAM-1/CD31
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背景
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Synonyms: CD31, CD31 antigen, EndoCAM, GPIIA, HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM 1, PECAM-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule
Background: PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.
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基因ID
- 18613
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NCBI登录号
- NP_001027550
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UniProt
- Q08481
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途径
- Regulation of Actin Filament Polymerization
抗原 See all CD31 (PECAM1) ELISA试剂盒
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