CD31 ELISA 试剂盒
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Quick Overview for CD31 ELISA 试剂盒 (ABIN921113)
抗原
See all CD31 (PECAM1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit to quantitate Mouse Pecam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E18-K590Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit
PECAM1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 人 AA 28-601 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Platelet/endothelial Cell Adhesion Molecule (PECAM1) ELISA KitPECAM1 适用: 小鼠 Colorimetric Sandwich ELISA 3.12-200 pg/mL
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.6%, 5.6%, 7.2%
Inter-Assay Precision (CV%): 7.9%, 7.1%, 7.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "The inhibition of Endostar on the angiogenesis and growth of gastrointestinal stromal tumor xenograft." in: Clinical and experimental medicine, Vol. 12, Issue 2, pp. 89-95, (2012) (PubMed).
: "Role of tumor-associated lymphatic endothelial cells in metastasis: a study of epithelial ovarian tumor in vitro." in: Cancer science, Vol. 101, Issue 3, pp. 679-85, (2010) (PubMed).
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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别名
- PECAM1
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背景
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Background: PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.
Gene Full Name: platelet/endothelial cell adhesion molecule 1
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基因ID
- 18613
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UniProt
- Q08481
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途径
- Regulation of Actin Filament Polymerization
抗原 See all CD31 (PECAM1) ELISA试剂盒
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