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LGALS1/Galectin 1 ELISA 试剂盒

This 小鼠 LGALS1/Galectin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 LGALS1/Galectin 1.
Rockland
产品编号 ABIN6720130
Supplier Product No.: koa0449
发货至: 中国

Quick Overview for LGALS1/Galectin 1 ELISA 试剂盒 (ABIN6720130)

抗原

See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒
LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0449

    Supplier

    Rockland

    原理

    Mouse Galectin-1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Galectin-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 5 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Galectin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Galectin-1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: A2-E135

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒

    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

    别名

    Galectin-1

    背景

    Synonyms: 14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding protein 14 kDa, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, GBP, HBL, HLBP14, HPL Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, LEG1_HUMAN, LGALS1, Putative MAPK-activating protein PM12, S-Lac lectin 1

    Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation. Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

    基因ID

    16852

    NCBI登录号

    NP_032521

    UniProt

    P16045

    途径

    Carbohydrate Homeostasis
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