KIT ELISA 试剂盒
Quick Overview for KIT ELISA 试剂盒 (ABIN6720112)
抗原
See all KIT ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0501
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Supplier
- Rockland
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原理
- Human KIT - SCFR Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human KIT. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Mast/stem Cell Growth Factor Receptor (KIT) ELISA Kit
KIT 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 人 AA 26-520 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 AA 25-527 Colorimetric Sandwich ELISA
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human KIT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KIT standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: sf21, Immunogen sequence: Q26-T520
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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别名
- KIT/SCFR
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背景
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Synonyms: CD117, CD117 antigen, EC 2.7.10.1, Mast/stem cell growth factor receptor precursor, PBT, Proto oncogene tyrosine protein kinase Kit, SCFR, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
Background: SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
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基因ID
- 3815
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NCBI登录号
- NP_000213
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UniProt
- P10721
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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