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ICAM-3/CD50 ELISA 试剂盒

This 人 ICAM-3/CD50 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ICAM-3/CD50.
Rockland
产品编号 ABIN6720105
Supplier Product No.: koa0575
发货至: 中国

Quick Overview for ICAM-3/CD50 ELISA 试剂盒 (ABIN6720105)

抗原

See all ICAM-3/CD50 (ICAM3) ELISA试剂盒
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

适用

  • 6
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0575

    Supplier

    Rockland

    原理

    Human ICAM-3 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human ICAM-3. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ICAM-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ICAM-3 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: Q30-H485, homodimer

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all ICAM-3/CD50 (ICAM3) ELISA试剂盒

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    别名

    ICAM-3

    背景

    Synonyms: CD50, CD50 antigen, CDW50, ICAM 3, ICAM R, ICAMR, Intercellular adhesion molecule 3, Intercellular adhesion molecule 3 precursor

    Background: Intercellular adhesion molecule 3(ICAM3) also known as CD50(Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

    基因ID

    3385

    NCBI登录号

    NP_002153

    UniProt

    P32942
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