ICAM-3/CD50 ELISA 试剂盒
Quick Overview for ICAM-3/CD50 ELISA 试剂盒 (ABIN6720105)
抗原
See all ICAM-3/CD50 (ICAM3) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0575
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Supplier
- Rockland
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原理
- Human ICAM-3 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human ICAM-3. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Intercellular Adhesion Molecule 3 (ICAM3) ELISA Kit
ICAM3 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 3 (ICAM3) ELISA KitICAM3 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 3 (ICAM3) ELISA KitICAM3 适用: 人 AA 30-485 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 3 (ICAM3) ELISA KitICAM3 适用: 人 Colorimetric Sandwich ELISA 65-16000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ICAM-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ICAM-3 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Q30-H485, homodimer
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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别名
- ICAM-3
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背景
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Synonyms: CD50, CD50 antigen, CDW50, ICAM 3, ICAM R, ICAMR, Intercellular adhesion molecule 3, Intercellular adhesion molecule 3 precursor
Background: Intercellular adhesion molecule 3(ICAM3) also known as CD50(Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.
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基因ID
- 3385
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NCBI登录号
- NP_002153
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UniProt
- P32942
抗原 See all ICAM-3/CD50 (ICAM3) ELISA试剂盒
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