ICAM-3/CD50 ELISA 试剂盒
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- 抗原 See all ICAM-3/CD50 (ICAM3) ELISA试剂盒
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 抗原表位
- AA 30-485
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ICAM-3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: Q30-H485
Immunogen sequence: homodimer - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO, Q30-H485
Immunogen sequence: homodimer - Featured
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Intercellular Adhesion Molecule 3 (ICAM3) ELISA Kit
ICAM3 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 3 (ICAM3) ELISA KitICAM3 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 3 (ICAM3) ELISA KitICAM3 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.
- 板类型
- Pre-coated
- 实验流程
- human ICAM-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-3 has been precoated onto 96-well plates. Standards(NSO, Q30-H485, homodimer) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-3 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ICAM-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ICAM-3 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 0.58, Standard deviation: 0.021, CV(%): 3.7
- Sample 2: n=16, Mean(ng/ml): 2.73, Standard deviation: 0.123, CV(%): 4.5
- Sample 3: n=16, Mean(ng/ml): 5.45, Standard deviation: 0.294, CV(%): 5.4,
- Sample 1: n=24, Mean(ng/ml): 0.68, Standard deviation: 0.04, CV(%): 5.9
- Sample 2: n=24, Mean(ng/ml): 3.16, Standard deviation: 0.215, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 6.24, Standard deviation: 0.443, CV(%): 7.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-?B signaling pathway." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 68, Issue 4, pp. 455-61, (2014) (PubMed).
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: "Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-?B signaling pathway." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 68, Issue 4, pp. 455-61, (2014) (PubMed).
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- 抗原 See all ICAM-3/CD50 (ICAM3) ELISA试剂盒
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 别名
- ICAM3 (ICAM3 产品)
- 别名
- CD50 ELISA Kit, CDW50 ELISA Kit, ICAM-R ELISA Kit, ICAM-3 ELISA Kit, ICAM3 ELISA Kit, intercellular adhesion molecule 3 ELISA Kit, ICAM3 ELISA Kit, Icam3 ELISA Kit
- 背景
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Protein Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
Background: Intercellular adhesion molecule 3(ICAM3) also known as CD50(Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.
Synonyms: Intercellular adhesion molecule 3,ICAM-3,CDw50,ICAM-R,CD50,ICAM3,
Full Gene Name: Intercellular adhesion molecule 3
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 3385
- UniProt
- P32942
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