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Cathepsin B ELISA 试剂盒

This 人 Cathepsin B ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Cathepsin B.
Rockland
产品编号 ABIN6720096
Supplier Product No.: koa0385
发货至: 中国

Quick Overview for Cathepsin B ELISA 试剂盒 (ABIN6720096)

抗原

See all Cathepsin B (CTSB) ELISA试剂盒
Cathepsin B (CTSB)

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0385

    Supplier

    Rockland

    原理

    Human Cathepsin B Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human Cathepsin B. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 5 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin B standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: R18-I339

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Cathepsin B (CTSB) ELISA试剂盒

    Cathepsin B (CTSB)

    别名

    Cathepsin B

    背景

    Synonyms: aAmyloid precursor protein secretase, APP secretase, APPS, CATB_HUMAN, Cathepsin B heavy chain, Cathepsin B1, CPSB, CTSB, Cysteine protease

    Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

    基因ID

    1508

    NCBI登录号

    NP_001899

    UniProt

    P07858

    途径

    Activation of Innate immune Response, Toll-Like Receptors Cascades
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