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Angiopoietin 1 ELISA 试剂盒

This 小鼠 Angiopoietin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Angiopoietin 1 in Cell Culture Supernatant, Plasma (EDTA - heparin) 和 Serum.
Rockland
产品编号 ABIN6720078
Supplier Product No.: koa0770
发货至: 中国

Quick Overview for Angiopoietin 1 ELISA 试剂盒 (ABIN6720078)

抗原

See all Angiopoietin 1 (ANGPT1) ELISA试剂盒
Angiopoietin 1 (ANGPT1)

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0770

    Supplier

    Rockland

    原理

    Mouse Angiopoietin-1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Angiopoietin-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Angiopoietin-1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: H16-F497

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Angiopoietin 1 (ANGPT1) ELISA试剂盒

    Angiopoietin 1 (ANGPT1)

    别名

    Angiopoietin-1

    背景

    Synonyms: 1110046O21Rik, AGP 1, AGP1, AGPT, ANG 1, ANG1, Angiopoietin1, ANGPT 1, ANGPT1, KIAA0004

    Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

    基因ID

    11600

    NCBI登录号

    NP_033770

    UniProt

    O08538

    途径

    RTK signaling, Glycosaminoglycan Metabolic Process
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