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FGF23 ELISA 试剂盒

This 小鼠 FGF23 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 FGF23.
Rockland
产品编号 ABIN6720064
Supplier Product No.: koa0823
发货至: 中国

Quick Overview for FGF23 ELISA 试剂盒 (ABIN6720064)

抗原

See all FGF23 ELISA试剂盒
FGF23 (Fibroblast Growth Factor 23 (FGF23))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0823

    Supplier

    Rockland

    原理

    Mouse FGF23 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse FGF23. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse FGF23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse FGF23 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: Y25-V251

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all FGF23 ELISA试剂盒

    FGF23 (Fibroblast Growth Factor 23 (FGF23))

    别名

    FGF23

    背景

    Synonyms: ADHR, FGF-23, Fgf23, FGF23_HUMAN, FGFN, Fibroblast growth factor 23, Fibroblast growth factor 23 C-terminal peptide, Fibroblast growth factor 23 precursor, HPDR2, HYPF, Phosphatonin, PHPTC, Tumor derived hypophosphatemia inducing factor, Tumor-derived hypophosphatemia-inducing factor

    Background: Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.

    基因ID

    64654

    NCBI登录号

    NP_073148

    UniProt

    Q9EPC2

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion
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