IL-37 ELISA 试剂盒

Production: Natural and recombinant human IL-37. There is no detectable cross-reactivity with other relevant proteins.

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

ELISA_Dilution: 15.6 pg/mL-1000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-37 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human IL-37 standard solution and each sample is measured in duplicate.

Standard: Expression system for standard: E.coli, Immunogen sequence: V46-D218

Synonyms: FIL1, FIL1 zeta, FIL1(ZETA), FIL1Z, IL 1 zeta, IL 1F7, IL 1H4, IL 1RP1, IL 1X, IL 1X protein, IL-1 zeta, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-1X, IL-37, IL1F7, IL1F7_HUMAN, IL1H4, IL1RP1, Interleukin 1 family member 7, interleukin 1 family member 7 (zeta), Interleukin 1 homolog 4, Interleukin 1 related protein, Interleukin 1 superfamily z, Interleukin 1 zeta, Interleukin 37, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, Interleukin-23

Background: Interleukin 37(IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.