IL-37 ELISA 试剂盒
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北京 101111
Quick Overview for IL-37 ELISA 试剂盒 (ABIN7671724)
抗原
See all IL-37 (IL37) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 31.25 pg/mL
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原理
- Human IL37 (Interleukin-37) QuickTest ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with IL37 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (When the sample content is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=19) undiluted ND-46pg/mL Healthy plasma (EDTA, Citrate , heparin) (n=20) undiluted ND-41pg/mL THP-1 cells were cultured in RPMI 1640 supplemented with 80 nM TPA, 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate and incubated overnight. Add cell lysis buffer(Catalogue No.:E050), the cell lysate solution (total protein concentration measured by BCA assay: 2.3 mg/mL) were assayed for levels of human IL-37. 1/2 dilution 350pg/mg(total protein) THP-1 cells were cultured in RPMI 1640 supplemented with 80 nM TPA, 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate and incubated overnight. The cells were then stimulated with 1 μg/mL LPS stimulation for 12 hours, add cell lysis buffer(Catalogue No.:E050) and cell lysate solution (total protein concentration measured by BCA assay: 2.1 mg/mL) were assayed for levels of human IL-37. 1/2 dilution 649pg/mg(total protein) 2. KD sample validation (Detect IL37-KD THP-1 cells): Sample Type Dilution Ratio Content Wild THP-1 cells were cultured in RPMI 1640 supplemented with 80 nM TPA, 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate and incubated overnight. The cells were then stimulated with 1 μg/mL LPS stimulation for 12 hours, add cell lysis buffer(Catalogue No.:E050) and cell lysate solution (total protein concentration measured by BCA assay: 2.1 mg/mL) were assayed for levels of human IL-37. 1/2 dilution 773pg/mg(total protein) THP-1 cells (+siRNA) were cultured in RPMI 1640 supplemented with 80 nM TPA, 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate and incubated overnight. The cells were then stimulated with 1 μg/mL LPS stimulation for 12 hours, add cell lysis buffer(Catalogue No.:E050) and cell lysate solution (total protein concentration measured by BCA assay: 2.1 mg/mL) were assayed for levels of human IL-37. 1/2 dilution 160pg/mg(total protein) Note:ND is lower than the sensitivity of the kit and was not detected 3. Verified recombinant protein: (It is a normal phenomenon that some proteins have weak detection signals or cannot be detected at all due to differences in tags, sequences or protein activities) A. E.coli-derived Human IL-37 protein Val46-Asp218
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灵敏度
- 18.75 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Cap/Det Ab (Ready to use
- blue)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Interleukin 37 (IL37) ELISA Kit
IL37 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 37 (IL37) ELISA KitIL37 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 37 (IL37) ELISA KitIL37 适用: 人 AA 46-218 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 37 (IL37) ELISA KitIL37 适用: 人 Colorimetric Sandwich ELISA 80 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 37 (IL37) ELISA KitIL37 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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实验流程
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- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- IL-37 (IL37) (Interleukin 37 (IL37))
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别名
- IL37
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背景
- Interleukin-37, IL-37, FIL1 zeta, IL-1X, Interleukin-1 family member 7, IL-1F7, Interleukin-1 homolog 4, IL-1H, IL-1H4, Interleukin-1 zeta, IL-1 zeta, Interleukin-1-related protein, IL-1RP1, IL37, FIL1Z, IL1F7, IL1H4, IL1RP1
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UniProt
- Q9NZH6
抗原 See all IL-37 (IL37) ELISA试剂盒
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