Betacellulin ELISA 试剂盒
Quick Overview for Betacellulin ELISA 试剂盒 (ABIN6720035)
抗原
See all Betacellulin (BTC) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0608
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Supplier
- Rockland
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原理
- Human Betacellulin - BTC Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Betacellulin. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Betacellulin (BTC) ELISA Kit
BTC 适用: 大鼠 Colorimetric Sandwich ELISA 9.375 pg/mL - 600 pg/mL Plasma, Serum, Tissue Homogenate
Betacellulin (BTC) ELISA KitBTC 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Betacellulin (BTC) ELISA KitBTC 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Betacellulin (BTC) ELISA KitBTC 适用: 人 Colorimetric Sandwich ELISA 30-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Betacellulin (BTC) ELISA KitBTC 适用: 人 AA 32-111 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Betacellulin (BTC) ELISA KitBTC 适用: 小鼠 AA 32-111 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Betacellulin (BTC) ELISA KitBTC 适用: 大鼠 AA 32-111 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human Betacellulin standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Betacellulin standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: D32-Y111
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Betacellulin (BTC)
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别名
- Betacellulin/BTC
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背景
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Synonyms: Betacellulin precursor, BTC, Probetacellulin
Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
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基因ID
- 685
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NCBI登录号
- NP_001303892
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UniProt
- P35070
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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