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Epiregulin ELISA 试剂盒

This 小鼠 Epiregulin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Epiregulin.
Rockland
产品编号 ABIN6720031
Supplier Product No.: koa0841
发货至: 中国

Quick Overview for Epiregulin ELISA 试剂盒 (ABIN6720031)

抗原

See all Epiregulin (EREG) ELISA试剂盒
Epiregulin (EREG)

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

125 pg/mL - 8000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    125 pg/mL

    Supplier Product No.

    koa0841

    Supplier

    Rockland

    原理

    Mouse Epiregulin Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Epiregulin. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse Epiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Epiregulin standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: V53-L101

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Epiregulin (EREG) ELISA试剂盒

    Epiregulin (EREG)

    别名

    Epiregulin

    背景

    Synonyms: Epiregulin, EPR, ER, Ereg, EREG_HUMAN, Proepiregulin

    Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

    基因ID

    13874

    NCBI登录号

    NP_031976

    UniProt

    Q61521

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
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