Thrombospondin 1 ELISA 试剂盒
Quick Overview for Thrombospondin 1 ELISA 试剂盒 (ABIN6720022)
抗原
See all Thrombospondin 1 (THBS1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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Supplier Product No.
- koa0540
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Supplier
- Rockland
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原理
- Human THBS1 - TSP1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human THBS1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Thrombospondin 1 (THBS1) ELISA Kit
THBS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 人 Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 大鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 小鼠 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Thrombospondin 1 (THBS1) ELISA KitWide Range THBS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitHigh Sensitivity THBS1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Tissue Homogenate
Thrombospondin 1 (THBS1) ELISA KitTHBS1 适用: Pig Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma
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应用备注
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ELISA_Dilution: 1.56 ng/mL-100 ng/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: N19-P1170
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Thrombospondin 1 (THBS1)
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别名
- THBS1/TSP1
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背景
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Synonyms: THBS 1, THBS, THBS1, Thrombospondin 1, Thrombospondin-1, Tsp 1, TSP, Tsp1, TSP1_HUMAN
Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
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基因ID
- 7057
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NCBI登录号
- NP_003237
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UniProt
- P07996
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途径
- Autophagy
抗原 See all Thrombospondin 1 (THBS1) ELISA试剂盒
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