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LBP ELISA 试剂盒

This 人 LBP ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 LBP.
Rockland
产品编号 ABIN6720021
Supplier Product No.: koa0747
发货至: 中国

Quick Overview for LBP ELISA 试剂盒 (ABIN6720021)

抗原

See all LBP ELISA试剂盒
LBP (Lipopolysaccharide Binding Protein (LBP))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

1.56 ng/mL - 100 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    1.56 ng/mL

    Supplier Product No.

    koa0747

    Supplier

    Rockland

    原理

    Human LBP Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human LBP. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 50 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, and 0.781 ng/mL human LBP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human LBP standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A26-V481

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all LBP ELISA试剂盒

    LBP (Lipopolysaccharide Binding Protein (LBP))

    别名

    LBP

    背景

    Synonyms: BPI fold containing family D member 2, Bpifd2, LBP, LBP_HUMAN, LBP1, Lipopolysaccharide binding protein, Lipopolysaccharide-binding protein, LPS binding protein, Ly88, MGC22233, OTTHUMP00000030965, RP23-407H16.4

    Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

    基因ID

    3929

    NCBI登录号

    NP_004130

    UniProt

    P18428

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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