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Complement Factor H ELISA 试剂盒

Name: Complement Factor H ELISA 试剂盒
SKU: ABIN6720016
Availability: OutOfStock

This 小鼠 Complement Factor H ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Complement Factor H.

产品编号 ABIN6720016

Supplier Product No.: koa0844

发货至: 中国

Quick Overview for Complement Factor H ELISA 试剂盒 (ABIN6720016)

抗原

See all Complement Factor H (CFH) ELISA试剂盒

Complement Factor H (CFH)

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.78 ng/mL - 50 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Discover our top product CFH ELISA Kit

最低检测浓度

0.78 ng/mL

Supplier Product No.

koa0844

Supplier

Rockland

原理

Mouse Complement H - CFH Sandwich ELISA Kit for Quantitative Detection

品牌

AccuSignal™

Analytical Method

Quantitative

特异性

Production: Natural and recombinant mouse CFH. There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 20 pg/mL

组件
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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应用备注

ELISA_Dilution: 0.78 ng/mL-50 ng/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CFH standard solution and each sample is measured in duplicate.

说明

Standard: Expression system for standard: NSO, Immunogen sequence: S875-V1252

样本量

100 μL

板类型

Pre-coated

限制

仅限研究用
储存条件

4 °C,-20 °C

储存方法

Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

有效期

12 months
抗原 See all Complement Factor H (CFH) ELISA试剂盒

Complement Factor H (CFH)

别名

Complement H/CFH

背景

Synonyms: adrenomedullin binding protein, age related maculopathy susceptibility 1, AHUS 1, AHUS1, AMBP 1, AMBP1, ARMD 4, ARMD4, ARMS 1, ARMS1, beta 1 H globulin, beta 1H, beta1H, CFAH_HUMAN, CFH, CFHL 3, CFHL3, Complement factor H, complement factor H isoform b, Factor H, factor H like 1, FH, FHL 1, FHL1, H factor 1 (complement)|H factor 1, H factor 2 (complement), HF 1, HF 2, HF, HF1, HF2, HUS, MGC88246
Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

基因ID

12628

NCBI登录号

NP_034018

UniProt

P06909

途径

Complement System, Cellular Response to Molecule of Bacterial Origin