KNG1 ELISA 试剂盒
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北京 101111
Quick Overview for KNG1 ELISA 试剂盒 (ABIN6720011)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0663
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Supplier
- Rockland
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原理
- Human Kininogen-1 - KNG1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q19-S644 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Kininogen 1 (KNG1) ELISA Kit
KNG1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Sandwich ELISA 47 ng/mL - 3000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 小鼠 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: Pig Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Kininogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Kininogen-1 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.9%, 5.1%, 5.8%
Inter-Assay Precision (CV%): 7.1%, 7.6%, 7.8% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- KNG1 (Kininogen 1 (KNG1))
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别名
- Kininogen-1/KNG1
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背景
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Background: Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins - high - molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
Gene Full Name: kininogen 1
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基因ID
- 3827
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NCBI登录号
- NP_000884
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UniProt
- P01042
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途径
- ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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