KNG1 ELISA 试剂盒
Quick Overview for KNG1 ELISA 试剂盒 (ABIN6720011)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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Supplier Product No.
- koa0663
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Supplier
- Rockland
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原理
- Human Kininogen-1 - KNG1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Kininogen-1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Kininogen 1 (KNG1) ELISA Kit
KNG1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Competition ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Plasma, Serum
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 大鼠 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: Pig Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 小鼠 Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 小鼠 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Plasma
Kininogen 1 (KNG1) ELISA KitHigh Sensitivity KNG1 适用: 人 Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kininogen 1 (KNG1) ELISA KitKNG1 适用: 人 AA 19-644 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Kininogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Kininogen-1 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Q19-S644
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- KNG1 (Kininogen 1 (KNG1))
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别名
- Kininogen-1/KNG1
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背景
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Synonyms: Alpha-2-thiol proteinase inhibitor, BDK, BK, BK, included, bradykinin, bradykinin, included, Fitzgerald factor, Fitzgerald factor, included, Flaujeac factor, included, High molecular weight kininogen, High molecular weight kininogen, included, HMWK, HMWK, included, Ile-Ser-Bradykinin, Kallidin I, Kallidin II, kininogen 1, KNG, KNG1, KNG1_HUMAN, LMWK, included, Low molecular weight growth-promoting factor, Low molecular weight kininogen, included, Williams factor, included, Williams-Fitzgerald-Flaujeac factor
Background: Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins - high - molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
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基因ID
- 3827
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NCBI登录号
- NP_000884
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UniProt
- P01042
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途径
- ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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