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CCL3 ELISA 试剂盒

This 小鼠 CCL3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CCL3 in Cell Culture Supernatant, Plasma (EDTA - heparin - citrate) 和 Serum. This ELISA Kit has been cited in 2+ publications.
Rockland
产品编号 ABIN6720004
Supplier Product No.: koa0258
发货至: 中国

Quick Overview for CCL3 ELISA 试剂盒 (ABIN6720004)

抗原

See all CCL3 ELISA试剂盒
CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

7.8 pg/mL - 500 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
  • 最低检测浓度

    7.8 pg/mL

    Supplier Product No.

    koa0258

    Supplier

    Rockland

    原理

    Mouse CCL3 - MIP1 alpha Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse CCL3. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence:  A24-A92

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • Wang, Huang, Lu, Liu, Mo: "Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation." in: Nature communications, Vol. 15, Issue 1, pp. 5176, (2024) (PubMed).

    Nian, Li, Ma, Li, Li, Zhang, Ohiolei, Li, Dai, Liu, Yan, Fu, Xiao, Jia: "Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice." in: PLoS neglected tropical diseases, Vol. 16, Issue 5, pp. e0010435, (2022) (PubMed).

  • 抗原 See all CCL3 ELISA试剂盒

    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

    别名

    CCL3/MIP1 alpha

    背景

    Synonyms: C C motif chemokine 3, CCL3, CCL3_HUMAN, Chemokine ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, Heparin binding chemotaxis protein, LD78-alpha, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP-1-alpha, MIP-1-alpha, MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

    Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

    基因ID

    20302

    NCBI登录号

    NP_035467

    UniProt

    P10855

    途径

    Cellular Response to Molecule of Bacterial Origin, Autophagy
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