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IL-18 ELISA 试剂盒

This 大鼠 IL-18 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 IL-18. This ELISA Kit has been cited in1 publication.
Rockland
产品编号 ABIN6719969
Supplier Product No.: koa0362
发货至: 中国

Quick Overview for IL-18 ELISA 试剂盒 (ABIN6719969)

抗原

See all IL-18 (IL18) ELISA试剂盒
IL-18 (IL18) (Interleukin 18 (IL18))

适用

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大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0362

    Supplier

    Rockland

    原理

    Rat IL-18 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant rat IL-18. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat IL-18 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: H37-S194

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • Zhou, Wang, Yang, Wu, Huan: "Spontaneous hypothermia ameliorated inflammation and neurologic deficit in rat cardiac arrest models following resuscitation." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 2127-2136, (2018) (PubMed).

  • 抗原 See all IL-18 (IL18) ELISA试剂盒

    IL-18 (IL18) (Interleukin 18 (IL18))

    别名

    IL-18

    背景

    Synonyms: Iboctadekin, IFN gamma inducing factor, IFN-gamma-inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL-1 gamma, IL-18, IL1 gamma, IL18, IL18 protein, IL18_HUMAN, IL1F4, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interferon gamma-inducing factor, Interleukin 1 gamma, Interleukin 18, Interleukin-1 gamma, Interleukin-18, Interleukin18, MGC12320

    Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

    基因ID

    29197

    NCBI登录号

    NP_062038

    UniProt

    P97636

    途径

    Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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