MMP3 ELISA 试剂盒
Quick Overview for MMP3 ELISA 试剂盒 (ABIN6719932)
抗原
See all MMP3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0270
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Supplier
- Rockland
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原理
- Mouse MMP-3 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse total MMP-3. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA Kit
MMP3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 人 AA 18-477 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 兔 Colorimetric Sandwich ELISA 2.19 ng/mL - 140 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin) to each empty well. It is recommended that each mouse MMP-3 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: Y18-C477
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))
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别名
- MMP-3
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背景
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Synonyms: CHDS6, matrix metallopeptidase 3 (stromelysin 1, progelatinase), Matrix metalloproteinase 3, Matrix metalloproteinase 3 preproprotein, Matrix metalloproteinase-3, MGC126102, MGC126103, MGC126104, MMP 3, MMP-3, MMP3, MMP3_HUMAN, Progelatinase, Proteoglycanase, SL 1, SL-1, SL1, STMY, STMY1, STR1, Stromelisin 1, Stromelysin 1, Stromelysin 1 progelatinase, Stromelysin-1, Transin 1, Transin-1
Background: Matrix metalloproteinase-3(MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase(MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54 % identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.
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基因ID
- 17392
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NCBI登录号
- NP_034939
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UniProt
- P28862
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