MMP3 ELISA 试剂盒
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北京 101111
Quick Overview for MMP3 ELISA 试剂盒 (ABIN411330)
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See all MMP3 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human MMP-3 ELISA Kit to quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Y18-C477Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is cross-reactivity with MMP-10 approximately 2 % , and no detectable cross-reactivity with other MMPs.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA Kit
MMP3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 AA 18-477 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3) ELISA KitMMP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.9%, 6.5%, 6.4%
Inter-Assay Precision (CV%): 6.2%, 6.8%, 6.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Nimbolide suppresses non-small cell lung cancer cell invasion and migration via manipulation of DUSP4 expression and ERK1/2 signaling." in: Biomedicine & pharmacotherapy, Vol. 92, pp. 340-346, (2017) (PubMed).
: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).
: "Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis: A Pilot Study." in: Cartilage, Vol. 8, Issue 4, pp. 384-390, (2017) (PubMed).
: "P2X7 mediates ATP-driven invasiveness in prostate cancer cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e114371, (2015) (PubMed).
: "?-Melanocyte-stimulating-hormone (?-MSH) modulates human chondrocyte activation induced by proinflammatory cytokines." in: BMC musculoskeletal disorders, Vol. 16, pp. 154, (2015) (PubMed).
: "Eotaxin-1 promotes prostate cancer cell invasion via activation of the CCR3-ERK pathway and upregulation of MMP-3 expression." in: Oncology reports, Vol. 31, Issue 5, pp. 2049-54, (2014) (PubMed).
: "Tumor necrosis factor-?-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo." in: European journal of cell biology, Vol. 94, Issue 1, pp. 12-21, (2014) (PubMed).
: "Vitiligo: a possible model of degenerative diseases." in: PLoS ONE, Vol. 8, Issue 3, pp. e59782, (2014) (PubMed).
: "Chitosan-plasmid DNA nanoparticles encoding small hairpin RNA targeting MMP-3 and -13 to inhibit the expression of dedifferentiation related genes in expanded chondrocytes." in: Journal of biomedical materials research. Part A, Vol. 102, Issue 2, pp. 373-80, (2013) (PubMed).
: "Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts." in: International journal of ophthalmology, Vol. 4, Issue 4, pp. 353-6, (2012) (PubMed).
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- MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))
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别名
- MMP3
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背景
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Background: Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54 % identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.
Gene Full Name: matrix metallopeptidase 3
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基因ID
- 4314
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UniProt
- P08254
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