SPARC ELISA 试剂盒
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- 抗原 See all SPARC ELISA试剂盒
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Breast Milk, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: AA517111, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, BM40, Cysteine rich protein, MGC128090, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and rich in cysteine, Secreted protein acidic cysteine rich (osteonectin), Secreted protein acidic cysteine rich, SPARC, SPRC, SPRC_HUMAN
Background: SPARC(secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I), 2) a cysteine- rich(domain II), 3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.
Gene Name: SPARC
Production: Natural and recombinant human SPARC. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: A18-I303
- Top Product
- Discover our top product SPARC ELISA Kit
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) ELISA Kit
SPARC 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) ELISA KitSPARC 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) ELISA KitSPARC 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) ELISA KitSPARC 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate, Urine
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) ELISA KitSPARC 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) ELISA KitSPARC 适用: 人 AA 18-303 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Milk, Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human SPARC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or human milk to each empty well. We recommend that each human SPARC standard solution and each sample is measured in duplicate.
ELISA Dilution: 0.78 ng/mL-50 ng/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all SPARC ELISA试剂盒
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- 别名
- SPARC (SPARC 产品)
- 别名
- ON ELISA Kit, AA517111 ELISA Kit, BM-40 ELISA Kit, hm:zeh0062 ELISA Kit, SPARC ELISA Kit, sparc ELISA Kit, secreted protein acidic and cysteine rich ELISA Kit, secreted acidic cysteine rich glycoprotein ELISA Kit, secreted protein, acidic, cysteine-rich (osteonectin) ELISA Kit, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog ELISA Kit, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog ELISA Kit, SPARC ELISA Kit, Sparc ELISA Kit, sparc ELISA Kit, sparc.L ELISA Kit, sparc.S ELISA Kit
- 基因ID
- 6678
- NCBI登录号
- NP_001296372
- UniProt
- P09486
- 途径
- Autophagy
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