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SHBG ELISA 试剂盒

This 人 SHBG ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 SHBG.
Rockland
产品编号 ABIN6719904
Supplier Product No.: koa0728
发货至: 中国

Quick Overview for SHBG ELISA 试剂盒 (ABIN6719904)

抗原

See all SHBG ELISA试剂盒
SHBG (Sex Hormone Binding Globulin (SHBG))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.031 nmol/L - 2 nmol/L

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    0.031 nmol/L

    Supplier Product No.

    koa0728

    Supplier

    Rockland

    原理

    Human SHBG Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human SHBG. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 0.001 nmol/L

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2nmol/L, 1nmol/L, 0.5nmol/L, 0.25nmol/L, 0.125nmol/L, 0.062nmol/L, 0.031nmol/L human SHBG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human SHBG standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L30-H402

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all SHBG ELISA试剂盒

    SHBG (Sex Hormone Binding Globulin (SHBG))

    别名

    SHBG

    背景

    Synonyms: ABP, Androgen binding protein, SBP, Sex hormone-binding globulin, Sex steroid binding protein, Sex steroid-binding protein, SHBG, SHBG_ HUMAN, TeBG, Testis specific androgen binding protein, Testis-specific androgen-binding protein, Testosterone binding beta globulin, Testosterone estradiol binding globulin, Testosterone estrogen binding globulin, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin

    Background: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12→p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

    基因ID

    6462

    NCBI登录号

    NP_001031

    UniProt

    P04278

    途径

    Hormone Transport
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