SHBG ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for SHBG ELISA 试剂盒 (ABIN2859324)
抗原
See all SHBG ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.063 nmol/L
-
原理
- Sandwich Human SHBG / Sex Hormone Binding Globulin ELISA Kit to quantitate Human SHBG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.001 nmol/L.
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: L30-H402Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 0.01 nmol/L
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Sex Hormone Binding Globulin (SHBG) ELISA Kit
SHBG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 小鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 人 Colorimetric Sandwich ELISA 15.625 pmol/L - 1000 pmol/L Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSmall Sample SHBG 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 3.0%, 2.5%, 2.9%
Inter-Assay Precision (CV%): 6.8%, 3.4%, 2.8% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- SHBG (Sex Hormone Binding Globulin (SHBG))
-
别名
- SHBG
-
背景
-
Background: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12→p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.
Gene Full Name: Sex hormone-binding globulin
-
基因ID
- 6462
-
UniProt
- P04278
-
途径
- Hormone Transport
抗原 See all SHBG ELISA试剂盒
-
