SHBG ELISA 试剂盒
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- 抗原 See all SHBG ELISA试剂盒
- SHBG (Sex Hormone Binding Globulin (SHBG))
- 抗原表位
- AA 30-402
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.031-2 nM/L
- 最低检测浓度
- 0.031 nM/L
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SHBG
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: L30-H402 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <0.001nmol/L
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: L30-H402 - Featured
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- Discover our top product SHBG ELISA Kit
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Sex Hormone Binding Globulin (SHBG) ELISA Kit
SHBG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 小鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSmall Sample SHBG 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Sex Hormone Binding Globulin (SHBG) ELISA KitSHBG 适用: 兔 Colorimetric Sandwich ELISA 25-400 pM/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 2 laminin G-like domains.
Tissue Specificity: Isoform 1 and isoform 2 are present in liver and testis.
- 板类型
- Pre-coated
- 实验流程
- human SHBG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SHBG has been precoated onto 96-well plates. Standards(NSO, L30-H402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SHBG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SHBG amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2nmol/L, 1nmol/L, 0.5nmol/L, 0.25nmol/L, 0.125nmol/L, 0.062nmol/L, 0.031nmol/L human SHBG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human SHBG standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(nmol/L): 0.19, Standard deviation: 0.009, CV(%): 4.5
- Sample 2: n=16, Mean(nmol/L): 0.76, Standard deviation: 0.043, CV(%): 5.6
- Sample 3: n=16, Mean(nmol/L): 1.4, Standard deviation: 0.095, CV(%): 6.8,
- Sample 1: n=24, Mean(nmol/L): 0.24, Standard deviation: 0.014, CV(%): 5.7
- Sample 2: n=24, Mean(nmol/L): 0.85, Standard deviation: 0.051, CV(%): 6
- Sample 3: n=24, Mean(nmol/L): 1.9, Standard deviation: 0.137, CV(%):7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all SHBG ELISA试剂盒
- SHBG (Sex Hormone Binding Globulin (SHBG))
- 别名
- SHBG (SHBG 产品)
- 别名
- ABP ELISA Kit, SBP ELISA Kit, TEBG ELISA Kit, Abpa ELISA Kit, shbgl ELISA Kit, wu:fb66h07 ELISA Kit, wu:fd55b08 ELISA Kit, zgc:123063 ELISA Kit, shbg ELISA Kit, sex hormone binding globulin ELISA Kit, sex hormone-binding globulin ELISA Kit, sex hormone binding globulin L homeolog ELISA Kit, SHBG ELISA Kit, Shbg ELISA Kit, shbg ELISA Kit, shbg.L ELISA Kit
- 背景
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Protein Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Background: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12-p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.
Synonyms: Sex hormone-binding globulin,SHBG,Sex steroid-binding protein,SBP,Testis-specific androgen-binding protein,ABP,Testosterone-estradiol-binding globulin,TeBG,Testosterone-estrogen-binding globulin,SHBG,
Full Gene Name: Sex hormone-binding globulin
Cellular Localisation: Secreted . In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.. - 基因ID
- 6462
- UniProt
- P04278
- 途径
- Hormone Transport
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