Kallikrein 12 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Kallikrein 12 ELISA 试剂盒 (ABIN6719901)
抗原
See all Kallikrein 12 (KLK12) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 156 pg/mL
-
Supplier Product No.
- koa0670
-
Supplier
- Rockland
-
原理
- Human KLK12 Sandwich ELISA Kit for Quantitative Detection
-
品牌
- AccuSignal™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: A18-N248 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Kallikrein-Related Peptidase 12 (KLK12) ELISA Kit
KLK12 适用: 人 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein-Related Peptidase 12 (KLK12) ELISA KitKLK12 适用: 人 AA 18-248 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
-
-
说明
-
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.
-
实验精密度
-
Intra-Assay Precision (CV%): 4.3%, 7.2%, 6%
Inter-Assay Precision (CV%): 4.5%, 7.2%, 6.9% -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))
-
别名
- KLK12
-
背景
-
Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.
Gene Full Name: kallikrein related peptidase 12
-
基因ID
- 43849
-
NCBI登录号
- NP_062544
-
UniProt
- Q9UKR0
-
途径
- Complement System
抗原 See all Kallikrein 12 (KLK12) ELISA试剂盒
-
