Osteoactivin ELISA 试剂盒
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Quick Overview for Osteoactivin ELISA 试剂盒 (ABIN6719897)
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See all Osteoactivin (GPNMB) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0737
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Supplier
- Rockland
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原理
- Human GPNMB - Osteoactivin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: K23-P486 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Osteoactivin (GPNMB) ELISA Kit
GPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 AA 23-486 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 AA 23-502 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Cells, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA 45 pg/mL - 12000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human GPNMB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GPNMB standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.7%, 7.7%, 7.7%
Inter-Assay Precision (CV%): 4.8%, 7.4%, 9.3% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Osteoactivin (GPNMB)
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别名
- GPNMB/Osteoactivin
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背景
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Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Gene Full Name: glycoprotein nmb
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基因ID
- 10457
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NCBI登录号
- NP_001005340
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UniProt
- Q14956
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