Osteoactivin ELISA 试剂盒
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- 抗原 See all Osteoactivin (GPNMB) ELISA试剂盒
- Osteoactivin (GPNMB)
- 抗原表位
- AA 23-502
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Osteoactivin/GPNMB
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: K23-N502 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: K23-N502 - Featured
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Osteoactivin (GPNMB) ELISA Kit
GPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 AA 22-486 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA 45-12.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Cells, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the PMEL/NMB family.
Tissue Specificity: In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. .
- 板类型
- Pre-coated
- 实验流程
- mouse Osteoactivin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Osteoactivin has been precoated onto 96-well plates. Standards(NSO, K23-N502) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Osteoactivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Osteoactivin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse Osteoactivin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Osteoactivin standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1174, Standard deviation: 64.57, CV(%): 5.5
- Sample 2: n=16, Mean(pg/ml): 3248, Standard deviation: 207.9, CV(%): 6.4
- Sample 3: n=16, Mean(pg/ml): 5532, Standard deviation: 271.1, CV(%): 4.9,
- Sample 1: n=24, Mean(pg/ml): 1354, Standard deviation: 82.6, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 3426, Standard deviation: 246.7, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 5840, Standard deviation: 315.4, CV(%): 5.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Osteoactivin (GPNMB) ELISA试剂盒
- Osteoactivin (GPNMB)
- 别名
- GPNMB (GPNMB 产品)
- 别名
- HGFIN ELISA Kit, NMB ELISA Kit, DC-HIL ELISA Kit, Dchil ELISA Kit, ipd ELISA Kit, glycoprotein nmb ELISA Kit, glycoprotein (transmembrane) nmb ELISA Kit, glycoprotein nmb L homeolog ELISA Kit, Gpnmb ELISA Kit, GPNMB ELISA Kit, gpnmb.L ELISA Kit
- 背景
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Protein Function: Could be a melanogenic enzyme. .
Background: Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Synonyms: Transmembrane glycoprotein NMB,DC-HIL,Dendritic cell-associated transmembrane protein,Osteoactivin,Gpnmb,Dchil, Hgfin, Nmb,
Full Gene Name: Transmembrane glycoprotein NMB
Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Endomembrane system, Single-pass type I membrane protein . Cytoplasmic vesicle membrane, Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures. - 基因ID
- 93695
- UniProt
- Q99P91
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