Osteoactivin ELISA 试剂盒
Quick Overview for Osteoactivin ELISA 试剂盒 (ABIN6719897)
抗原
See all Osteoactivin (GPNMB) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0737
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Supplier
- Rockland
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原理
- Human GPNMB - Osteoactivin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human GPNMB. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Osteoactivin (GPNMB) ELISA Kit
GPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 AA 22-486 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 AA 23-502 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 小鼠 Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA 45-12.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Osteoactivin (GPNMB) ELISA KitGPNMB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Cells, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human GPNMB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GPNMB standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: A22-P486
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Osteoactivin (GPNMB)
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别名
- GPNMB/Osteoactivin
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背景
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Synonyms: Gpnmb, GPNMB_HUMAN, Transmembrane glycoprotein HGFIN, Transmembrane glycoprotein NMB
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
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基因ID
- 10457
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NCBI登录号
- NP_001005340
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UniProt
- Q14956
抗原 See all Osteoactivin (GPNMB) ELISA试剂盒
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