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TLR3 ELISA 试剂盒

This 人 TLR3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 TLR3.
Rockland
产品编号 ABIN6719884
Supplier Product No.: koa0547
发货至: 中国

Quick Overview for TLR3 ELISA 试剂盒 (ABIN6719884)

抗原

See all TLR3 ELISA试剂盒
TLR3 (Toll-Like Receptor 3 (TLR3))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0547

    Supplier

    Rockland

    原理

    Human TLR3 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human TLR3. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TLR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TLR3 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: K27-S711

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all TLR3 ELISA试剂盒

    TLR3 (Toll-Like Receptor 3 (TLR3))

    别名

    TLR3

    背景

    Synonyms: CD_antigen=CD283, CD283, IIAE2, Toll like receptor 3

    Background: Toll-like receptor 3(TLR3) also known as CD283(cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA(dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.

    基因ID

    7098

    NCBI登录号

    NP_003256

    UniProt

    O15455

    途径

    TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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