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P-Cadherin ELISA 试剂盒

This 人 P-Cadherin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 P-Cadherin.
Rockland
产品编号 ABIN6719852
Supplier Product No.: koa0382
发货至: 中国

Quick Overview for P-Cadherin ELISA 试剂盒 (ABIN6719852)

抗原

See all P-Cadherin (CDH3) ELISA试剂盒
P-Cadherin (CDH3)

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0382

    Supplier

    Rockland

    原理

    Human P-Cadherin Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human P-Cadherin. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 2 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human P-Cadherin standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: D108-G654

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all P-Cadherin (CDH3) ELISA试剂盒

    P-Cadherin (CDH3)

    别名

    P-Cadherin

    背景

    Synonyms: AI385538, CADH3_HUMAN, Cadherin 3, Cadherin 3 precursor, Cadherin 3 type 1, Cadherin-3, Cadp, Calcium dependent adhesion protein placental, CDH 3, CDH3, CDH3 protein, CDHP, HJMD, P cadherin (placental), P-cadherin, PCAD, Placental cadherin

    Background: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

    基因ID

    1001

    NCBI登录号

    NP_001304124

    UniProt

    P22223
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