P-Cadherin ELISA 试剂盒
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- 抗原 See all P-Cadherin (CDH3) ELISA试剂盒
- P-Cadherin (CDH3)
- 抗原表位
- AA 100-647
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse P-Cadherin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: E100-G647 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: E100-G647 - Featured
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P-Cadherin (CDH3) ELISA Kit
CDH3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
P-Cadherin (CDH3) ELISA KitCDH3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
P-Cadherin (CDH3) ELISA KitCDH3 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 AA 108-654 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse P-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for P-Cadherin has been precoated onto 96-well plates. Standards(NSO, E100-G647) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Cadherin amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse P-Cadherin standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 433, Standard deviation: 27.28, CV(%): 6.3
- Sample 2: n=16, Mean(pg/ml): 1314, Standard deviation: 88.04, CV(%): 6.7
- Sample 3: n=16, Mean(pg/ml): 2658, Standard deviation: 180.74, CV(%): 6.8,
- Sample 1: n=24, Mean(pg/ml): 437, Standard deviation: 31.90, CV(%): 7.3
- Sample 2: n=24, Mean(pg/ml): 1356, Standard deviation: 103.06, CV(%): 7.6
- Sample 3: n=24, Mean(pg/ml): 2713, Standard deviation: 211.61, CV(%): 7.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all P-Cadherin (CDH3) ELISA试剂盒
- P-Cadherin (CDH3)
- 别名
- CDH3 (CDH3 产品)
- 别名
- CDH3 ELISA Kit, Cadherin-3 ELISA Kit, AI385538 ELISA Kit, Cadp ELISA Kit, Cdhp ELISA Kit, P-cadherin ELISA Kit, Pcad ELISA Kit, CDHP ELISA Kit, HJMD ELISA Kit, PCAD ELISA Kit, E-Cad ELISA Kit, E-cadherin ELISA Kit, EP-cadherin ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, cdh1 ELISA Kit, cdh1-b ELISA Kit, cdh3 ELISA Kit, cdhc-A ELISA Kit, cdhp ELISA Kit, hjmd ELISA Kit, l-cam ELISA Kit, lcam ELISA Kit, pcad ELISA Kit, uvo ELISA Kit, uvomorulin ELISA Kit, xb-cad ELISA Kit, xcdh1 ELISA Kit, CDH15 ELISA Kit, K-CAM ELISA Kit, cadherin 3 ELISA Kit, cadherin 3 L homeolog ELISA Kit, Cadherin-3 ELISA Kit, CDH3 ELISA Kit, Cdh3 ELISA Kit, cdh3.L ELISA Kit, cdh-3 ELISA Kit
- 背景
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Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.
Background: P-Cadherin, also known as Cadherin-3(CDH3), is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. Cells expressing CDH3 also adhered to one another more tightly than the parental cell line.
Synonyms: Cadherin-3,Placental cadherin,P-cadherin,Cdh3,Cdhp,
Full Gene Name: Cadherin-3
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 12560
- UniProt
- P10287
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