NUCB2 ELISA 试剂盒
Quick Overview for NUCB2 ELISA 试剂盒 (ABIN6719848)
抗原
See all NUCB2 (NES1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0652
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Supplier
- Rockland
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原理
- Human Nesfatin-1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse CCL8. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Nesfatin-1 (NES1) ELISA Kit
NES1 适用: 人 Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitSmall Sample NES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Nesfatin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Nesfatin-1 standard solution and each sample be measured in duplicate. Seal the plate with the cover and incubate at 37 °C for 90 min.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: V25-L106
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- NUCB2 (NES1) (Nesfatin-1 (NES1))
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别名
- Nesfatin-1
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背景
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Synonyms: DNA binding protein NEFA, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NEFA, Nefastin 1, Nefastin-1, Nefastin1, Nucb2, NUCB2_HUMAN, Nucleobindin-2, Nucleobinding 2, Prepronefastin, Prepronesfatin
Background: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2(NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
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基因ID
- 4925
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NCBI登录号
- NP_005004
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UniProt
- P80303
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