NUCB2 ELISA 试剂盒
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Quick Overview for NUCB2 ELISA 试剂盒 (ABIN4881312)
抗原
See all NUCB2 (NES1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Nesfatin EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This kit detects human, mouse and rat 82aa Nesfatin protein. No other active isoforms have been reported.
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灵敏度
- 400 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Nesfatin-1 (NES1) ELISA Kit
NES1 适用: 人 Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitSmall Sample NES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).
: "The relation of serum nesfatin-1 level with metabolic and clinical parameters in obese and healthy children." in: Pediatric diabetes, Vol. 14, Issue 3, pp. 189-95, (2013) (PubMed).
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- NUCB2 (NES1) (Nesfatin-1 (NES1))
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别名
- Nesfatin
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背景
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Gene Symbols: NUCB2, NEFA
Background: Obesity, which is characterized by excessive accumulation of adipose tissue in the body, has become one of the greatest public health challenges. Obesity is not only associated with health problems linked to increased weight-dependent pressure overload on lung, joints and bones, but also a important risk factor for life-threatening diseases such as cardiovascular diseases, type 2 diabetes and certain cancers.
Nesfatin is a naturally occurring hormone produced by brain cells. Japanese scientists discovered in 2006 that it is responsible for regulating appetite and producing body fat. Excess Nesfatin in the brain leads to a loss of appetite, less frequent hunger, a "sense of fullness", and a drop in body fat and weight. A lack of Nesfatin in the brain leads to an increase of appetite, more frequent episodes of hunger, an increase of body fat and weight, and the inability to "feel full." This latter condition can be artificially induced by injecting an anti-Nesfatin antibody into the brain.
The receptors of Nesfatin within the brain are not completely understood, although they are thought to be contained in the hypothalamus and in the solitary nucleus, where Nesfatin is believed to produced via peroxisome proliferator-activated receptors (PPARs).Protein Name (Synonyms): Nucleobindin-2 (DNA-binding protein NEFA) (Gastric cancer antigen Zg4) (Prepronesfatin) [Cleaved into: Nesfatin-1]
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基因ID
- 4925
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UniProt
- P80303
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