NUCB2 ELISA 试剂盒
(2 references)-
- 抗原 See all NUCB2 (NES1) ELISA试剂盒
- NUCB2 (NES1) (Nesfatin-1 (NES1))
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适用
- 人, 大鼠, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.1-1.000 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Nesfatin EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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This kit detects human, mouse and rat 82aa Nesfatin protein. No other active isoforms have been reported.
- 灵敏度
- 400 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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- Discover our top product NES1 ELISA Kit
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Nesfatin-1 (NES1) ELISA Kit
NES1 适用: 人 Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 大鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitNES1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nesfatin-1 (NES1) ELISA KitNES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nesfatin-1 (NES1) ELISA KitSmall Sample NES1 适用: 小鼠 Colorimetric Competition ELISA 493.8 pg/mL - 40000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 2X / Rat: 2X
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- 有效期
- 6 months
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-
: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).
: "The relation of serum nesfatin-1 level with metabolic and clinical parameters in obese and healthy children." in: Pediatric diabetes, Vol. 14, Issue 3, pp. 189-95, (2013) (PubMed).
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: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." in: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).
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- 抗原 See all NUCB2 (NES1) ELISA试剂盒
- NUCB2 (NES1) (Nesfatin-1 (NES1))
- 别名
- Nesfatin (NES1 产品)
- 别名
- nucleobindin-2 ELISA Kit, nucleobindin 2 ELISA Kit, NUCB2 ELISA Kit, Nucb2 ELISA Kit
- 基因ID
- 4925
- UniProt
- P80303
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