MADCAM1 ELISA 试剂盒
Quick Overview for MADCAM1 ELISA 试剂盒 (ABIN6719835)
抗原
See all MADCAM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 125 pg/mL
-
Supplier Product No.
- koa0801
-
Supplier
- Rockland
-
原理
- Mouse MADCAM-1 Sandwich ELISA Kit for Quantitative Detection
-
品牌
- AccuSignal™
-
Analytical Method
- Quantitative
-
特异性
-
Production: Natural and recombinant mouse MADCAM-1. There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
-
-
-
-
应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse MADCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse MADCAM-1 standard solution and each sample be measured in duplicate.
-
说明
-
Standard: Expression system for standard: NSO, Immunogen sequence: Q22-T365
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
-
别名
- MADCAM-1
-
背景
-
Synonyms: Addressin mucosal, hMAdCAM 1, hMAdCAM-1, MACAM1, MADCA_HUMAN, MAdCAM 1, MAdCAM-1, Madcam1, Mucosal addressin cell adhesion molecule 1, Mucosal vascular addressin cell adhesion molecule 1
Background: MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4 / beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
-
基因ID
- 17123
-
NCBI登录号
- NP_038619
-
UniProt
- Q61826
抗原 See all MADCAM1 ELISA试剂盒
-