MADCAM1 ELISA 试剂盒
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- 抗原 See all MADCAM1 ELISA试剂盒
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
- 抗原表位
- AA 22-365
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MADCAM-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Q22-T365 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: Q22-T365 - Featured
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Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA Kit
MADCAM1 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA KitMADCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas. .
- 板类型
- Pre-coated
- 实验流程
- mouse MADCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MADCAM-1 has been precoated onto 96-well plates. Standards(NSO, Q22-T365) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MADCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MADCAM-1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse MADCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MADCAM-1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 853, Standard deviation: 53.74, CV(%): 6.3
- Sample 2: n=16, Mean(pg/ml): 2254, Standard deviation: 133, CV(%): 5.9
- Sample 3: n=16, Mean(pg/ml): 3827, Standard deviation: 183.7, CV(%): 4.8,
- Sample 1: n=24, Mean(pg/ml): 976, Standard deviation: 75.15, CV(%): 7.7
- Sample 2: n=24, Mean(pg/ml): 2486, Standard deviation: 164.1, CV(%): 6.6
- Sample 3: n=24, Mean(pg/ml): 4127, Standard deviation: 210.5, CV(%): 5.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all MADCAM1 ELISA试剂盒
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
- 别名
- MADCAM1 (MADCAM1 产品)
- 别名
- MACAM1 ELISA Kit, AV211525 ELISA Kit, mucosal vascular addressin cell adhesion molecule 1 ELISA Kit, MADCAM1 ELISA Kit, Madcam1 ELISA Kit
- 背景
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Protein Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. .
Background: MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4 / beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
Synonyms: Mucosal addressin cell adhesion molecule 1,MAdCAM-1, mMAdCAM-1,Madcam1,
Full Gene Name: Mucosal addressin cell adhesion molecule 1
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 17123
- UniProt
- Q61826
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