Angiotensin I Converting Enzyme 1 ELISA 试剂盒
Quick Overview for Angiotensin I Converting Enzyme 1 ELISA 试剂盒 (ABIN6719828)
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See all Angiotensin I Converting Enzyme 1 (ACE) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0337
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Supplier
- Rockland
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原理
- Human ACE Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human ACE. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA Kit
ACE 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cerebrospinal Fluid, Plasma, Serum
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 绵羊 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 豚鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA KitACE 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human ACE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin) or saliva to each empty well. We recommend that each human ACE standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: sf2NSO, Immunogen sequence: L30-L1261
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
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别名
- ACE
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背景
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Synonyms: ACE, ACE_HUMAN, ACE1, Angiotensin converting enzyme somatic isoform, Angiotensin I converting enzyme (peptidyl dipeptidase A) 1, Angiotensin-converting enzyme, Carboxycathepsin, CD143, CD143 antigen, DCP, DCP1, Dipeptidyl carboxypeptidase 1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3, Peptidase P, soluble form, Testicular ECA
Background: Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
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基因ID
- 1636
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NCBI登录号
- NP_000780
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UniProt
- B4DKH4
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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