RAGE ELISA 试剂盒
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Quick Overview for RAGE ELISA 试剂盒 (ABIN6719822)
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See all RAGE (AGER) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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Supplier Product No.
- koa0494
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Supplier
- Rockland
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原理
- Human Rage Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q24-A344 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA Kit
AGER 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 AA 24-344 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 小鼠 AA 24-342 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 大鼠 AA 24-342 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Advanced Glycosylation End Product-Specific Receptor (AGER) ELISA KitAGER 适用: 人 Colorimetric Sandwich ELISA 3 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Rage standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Rage standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 6.6%, 4.4%, 7.2%
Inter-Assay Precision (CV%): 6.7%, 5.5%, 7.5% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
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别名
- Rage
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背景
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Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as ""AGER"". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
Gene Full Name: advanced glycosylation end-product specific receptor
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基因ID
- 177
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NCBI登录号
- NP_001127
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UniProt
- Q15109
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途径
- Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
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