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RAGE ELISA 试剂盒

AGER 适用: 大鼠 AA 24-342 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1672873
发货至: 中国
  • 抗原 See all RAGE (AGER) ELISA试剂盒
    RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
    抗原表位
    AA 24-342
    适用
    • 7
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78-5000 pg/mL
    最低检测浓度
    78 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat RAGE
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: Q24-A342
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: Q24-A342
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    Tissue Specificity: Endothelial cells and cardiomyocytes. .

    板类型
    Pre-coated
    实验流程
    rat RAGE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RAGE has been precoated onto 96-well plates. Standards(NSO, Q24-A342) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RAGE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RAGE amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL rat RAGE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat RAGE standard solution and each sample is measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 592, Standard deviation: 36.1, CV(%): 6.1
    • Sample 2: n=16, Mean(pg/ml): 1590, Standard deviation: 84.27, CV(%): 5.3
    • Sample 3: n=16, Mean(pg/ml): 3161, Standard deviation: 180.2, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 633, Standard deviation: 46.21, CV(%): 7.3
    • Sample 2: n=24, Mean(pg/ml): 1386, Standard deviation: 88.71, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 3155, Standard deviation: 227.16, CV(%): 7.2
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Feng, Zhu, Zhang, Jia, Cheng, Ding, Zhu et al.: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).

  • 抗原 See all RAGE (AGER) ELISA试剂盒
    RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
    别名
    AGER (AGER 产品)
    别名
    RAGE ELISA Kit, AGER ELISA Kit, advanced glycosylation end-product specific receptor ELISA Kit, advanced glycosylation end product-specific receptor ELISA Kit, MAPK/MAK/MRK overlapping kinase ELISA Kit, AGER ELISA Kit, Ager ELISA Kit, LOC719012 ELISA Kit
    背景

    Protein Function: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides (By similarity). Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. .

    Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as AGER. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB).

    Synonyms: Advanced glycosylation end product-specific receptor,Receptor for advanced glycosylation end products,Ager,Rage,

    Full Gene Name: Advanced glycosylation end product-specific receptor

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    基因ID
    81722
    UniProt
    Q63495
    途径
    Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
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