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RAGE ELISA 试剂盒

This 人 RAGE ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 RAGE.
Rockland
产品编号 ABIN6719822
Supplier Product No.: koa0494
发货至: 中国

Quick Overview for RAGE ELISA 试剂盒 (ABIN6719822)

抗原

See all RAGE (AGER) ELISA试剂盒
RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

78 pg/mL - 5000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    78 pg/mL

    Supplier Product No.

    koa0494

    Supplier

    Rockland

    原理

    Human Rage Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human Rage. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Rage standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Rage standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: Q24-A344

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all RAGE (AGER) ELISA试剂盒

    RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

    别名

    Rage

    背景

    Synonyms: Advanced glycosylation end product specific receptor, Advanced glycosylation end product-specific receptor, Ager, DAMA 358M23.4, LE 9211 A antigen, MAPK MAK MRK overlapping kinase, MGC22357, MOK, OTTHUMP00000174885, RAGE 1, RAGE isoform NtRAGE-delta, RAGE isoform sRAGE-delta, RAGE_HUMAN, RAGE1, Receptor for advanced glycation end products, receptor for advanced glycation end-products variant 20, Receptor for advanced glycosylation end products, Renal tumor antigen, Renal tumor antigen 1

    Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as ""AGER"". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).

    基因ID

    177

    NCBI登录号

    NP_001127

    UniProt

    B4DNX3

    途径

    Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
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