CA9 ELISA 试剂盒
Quick Overview for CA9 ELISA 试剂盒 (ABIN6719792)
抗原
See all CA9 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0392
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Supplier
- Rockland
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原理
- Human CA9 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human CA9. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Carbonic Anhydrase IX (CA9) ELISA Kit
CA9 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 人 AA 59-414 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 AA 32-390 Colorimetric Sandwich ELISA
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 人 Colorimetric Sandwich ELISA 0.2-50 pg/mL Cell Culture Supernatant, Plasma, Serum
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CA9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human CA9 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: P59-D414
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CA9 (Carbonic Anhydrase IX (CA9))
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别名
- CA9
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背景
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Synonyms: CA-IX, CA9, CAH9_HUMAN, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated protein G250, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250
Background: Carbonic anhydrase 9(CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
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基因ID
- 768
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NCBI登录号
- NP_001207
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UniProt
- Q16790
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