CA9 ELISA 试剂盒
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- 抗原 See all CA9 ELISA试剂盒
- CA9 (Carbonic Anhydrase IX (CA9))
- 抗原表位
- AA 59-414
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA9
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: P59-D414 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: P59-D414 - Featured
- Discover our best selling CA9 ELISA Kit
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- Discover our top product CA9 ELISA Kit
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Carbonic Anhydrase IX (CA9) ELISA Kit
CA9 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 AA 32-390 Colorimetric Sandwich ELISA
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Carbonic Anhydrase IX (CA9) ELISA KitCA9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
- 板类型
- Pre-coated
- 实验流程
- human CA9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CA9 has been precoated onto 96-well plates. Standards(NSO, P59-D414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CA9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CA9 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CA9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CA9 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 152, Standard deviation: 6.4, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 354, Standard deviation: 15.9, CV(%): 4.5
- Sample 3: n=16, Mean(pg/ml): 687, Standard deviation: 31.6, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 158, Standard deviation: 11.1, CV(%): 7
- Sample 2: n=24, Mean(pg/ml): 361, Standard deviation: 24.5, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 637, Standard deviation: 41.4, CV(%): 6.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CA9 ELISA试剂盒
- CA9 (Carbonic Anhydrase IX (CA9))
- 别名
- CA9 (CA9 产品)
- 别名
- CA9 ELISA Kit, CAIX ELISA Kit, Ca9 ELISA Kit, MN/CA9 ELISA Kit, MN ELISA Kit, carbonic anhydrase 9 ELISA Kit, CA9 ELISA Kit, CAH9 ELISA Kit, Car9 ELISA Kit
- 背景
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Protein Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. .
Background: Carbonic anhydrase 9(CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
Synonyms: Carbonic anhydrase 9,4.2.1.1,Carbonate dehydratase IX,Carbonic anhydrase IX,CA-IX,CAIX,Membrane antigen MN,P54/58N,Renal cell carcinoma-associated antigen G250,RCC-associated antigen G250,pMW1,CA9,G250, MN,
Full Gene Name: Carbonic anhydrase 9
Cellular Localisation: Nucleus . Nucleus, nucleolus . Cell membrane, Single-pass type I membrane protein . Cell projection, microvillus membrane, Single-pass type I membrane protein . Found on the surface microvilli and in the nucleus, particularly in nucleolus. - 基因ID
- 768
- UniProt
- Q16790
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