Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA 试剂盒
Quick Overview for Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA 试剂盒 (ABIN6719787)
抗原
See all Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 7.8 pg/mL
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Supplier Product No.
- koa0320
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Supplier
- Rockland
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原理
- Human TNFsR I Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human TNFsR. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA Kit
sTNF-R1 适用: 人 Colorimetric Sandwich ELISA 78.125-5000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA KitsTNF-R1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA KitsTNF-R1 适用: 人 AA 30-211 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA KitsTNF-R1 适用: 人 Colorimetric Sandwich ELISA 13-800 pg/mL Cell Lysate, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TNFsRI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. We recommend that each human TNF s RI standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: L30-T211
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1)
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别名
- TNFsR I
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背景
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Synonyms: CD120a, FPF, p55, p55 R, p60, p65, TBP1, TBPI, TNF R, TNF R I, TNF R55, TNF-R1, TNF-RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRI, TNFRSF1A, TNR1A_HUMAN, Tumor necrosis factor alpha receptor, Tumor necrosis factor binding protein 1, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor receptor type I, Tumor necrosis factor-binding protein 1
Background: TNFsRI (also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors(TNF). In contrast, the p60(TNFRSF1A) and p80(TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain(PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL(TNFRSF10A), CD40, and FAS(TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor(the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.
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基因ID
- 7132
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NCBI登录号
- NP_001056
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UniProt
- P19438
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