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Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA 试剂盒

sTNF-R1 适用: 人 AA 30-211 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
产品编号 ABIN921076
发货至: 中国
  • 抗原 See all Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA试剂盒
    Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1)
    抗原表位
    AA 30-211
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    7.8-500 pg/mL
    最低检测浓度
    7.8 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFsR I
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO,L30-T211
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <1pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: L30-T211
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 1 death domain.

    板类型
    Pre-coated
    实验流程
    human TNFsRIELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFsRI has been precoated onto 96-well plates. Standards (NSO,L30-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFsRI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFsRI amount of sample captured in plate.
    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 74, Standard deviation: 2.52, CV(%): 3.4
    • Sample 2: n=16, Mean(pg/ml): 133, Standard deviation: 6.52, CV(%): 4.9
    • Sample 3: n=16, Mean(pg/ml): 261, Standard deviation: 14.9, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 85, Standard deviation: 3.91, CV(%): 4.6
    • Sample 2: n=24, Mean(pg/ml): 156, Standard deviation: 7.96, CV(%): 5.1
    • Sample 3: n=24, Mean(pg/ml): 278, Standard deviation: 18.07, CV(%): 6.5
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1) ELISA试剂盒
    Soluble Tumor Necrosis Factor Receptor Type 1 (sTNF-R1)
    别名
    TNFsRI (sTNF-R1 产品)
    别名
    CD120a ELISA Kit, FPF ELISA Kit, MS5 ELISA Kit, TBP1 ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR1-d2 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, p55 ELISA Kit, p55-R ELISA Kit, p60 ELISA Kit, TNF receptor superfamily member 1A ELISA Kit, TNFRSF1A ELISA Kit
    背景

    Protein Function: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

    Background: TNFsRI(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors(TNF). In contrast, the p60(TNFRSF1A) and p80(TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain(PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL(TNFRSF10A), CD40, and FAS(TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor(the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.

    Synonyms: Tumor necrosis factor receptor superfamily member 1A,Tumor necrosis factor receptor 1,TNF-R1,Tumor necrosis factor receptor type I,TNF-RI,TNFR-I,p55,p60,CD120a,Tumor necrosis factor receptor superfamily member 1A, membrane form,Tumor necrosis factor-binding protein 1,TBPI,TNFRSF1A,TNFAR, TNFR1,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 1A

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Golgi apparatus membrane, Single- pass type I membrane protein . Secreted . A secreted form is produced through proteolytic processing.
    基因ID
    7132
    UniProt
    P19438
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