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VEGFB ELISA 试剂盒

This 小鼠 VEGFB ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 VEGFB.
Rockland
产品编号 ABIN6719783
Supplier Product No.: koa0852
发货至: 中国

Quick Overview for VEGFB ELISA 试剂盒 (ABIN6719783)

抗原

See all VEGFB ELISA试剂盒
VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (heparin), Serum
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0852

    Supplier

    Rockland

    原理

    Mouse VEGF-B Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse VEGF-B. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 2 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 31.2pg/mL-2000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse VEGF-B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse VEGF-B standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: sf21, Immunogen sequence: Q20-A207&Q20-R148

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all VEGFB ELISA试剂盒

    VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

    别名

    VEGF-B

    背景

    Synonyms: Vascular endothelial growth factor B, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor, Vegfb, VEGFB_HUMAN, VEGFL, VRF

    Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

    基因ID

    22340

    NCBI登录号

    NP_001172093

    UniProt

    P49766

    途径

    RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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