Midkine ELISA 试剂盒
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- 抗原 See all Midkine (MDK) ELISA试剂盒
- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Amphiregulin-associated protein, ARAP, Mdk, Midgestation and kidney protein, Midkine, MK, MK_HUMAN, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein
Background: Midkine(MK or MDK) also known as neurite growth-promoting factor 2(NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46 % homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPζ), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase(ALK) and syndecans is considered to be its receptor.
Gene Name: MDK
Production: Natural and recombinant human Midkine. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: K23-D143
- Top Product
- Discover our top product MDK ELISA Kit
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kit
MDK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK 适用: 人 AA 23-143 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human Midkine standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Midkine standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 78pg/mL-5000pg/mL
Other Performance Data: DOE: 2-8° C - 5/9/18 -20° C - 11/10/18
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all Midkine (MDK) ELISA试剂盒
- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
- 别名
- Midkine (MDK 产品)
- 别名
- ARAP ELISA Kit, MK ELISA Kit, NEGF2 ELISA Kit, Mek ELISA Kit, MK-B ELISA Kit, PTF-alpha-1 ELISA Kit, mdk ELISA Kit, midkine ELISA Kit, negf2 ELISA Kit, RIHB ELISA Kit, cb518 ELISA Kit, fb79b05 ELISA Kit, fj33b02 ELISA Kit, fj48a12 ELISA Kit, mdk2 ELISA Kit, plei2 ELISA Kit, wu:fb79b05 ELISA Kit, wu:fj33b02 ELISA Kit, wu:fj48a12 ELISA Kit, MK-A ELISA Kit, PTF-alpha-2 ELISA Kit, X-PTF-alpha1 ELISA Kit, XMK ELISA Kit, mdk-a ELISA Kit, mdk-b ELISA Kit, midkine ELISA Kit, midkine L homeolog ELISA Kit, midkine (neurite growth-promoting factor 2) ELISA Kit, midkine b ELISA Kit, midkine S homeolog ELISA Kit, MDK ELISA Kit, Mdk ELISA Kit, mdk.L ELISA Kit, mdkb ELISA Kit, mdk.S ELISA Kit
- 基因ID
- 4192
- NCBI登录号
- NP_001012333
- UniProt
- P21741
- 途径
- RTK signaling, M Phase, Skeletal Muscle Fiber Development
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