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Renin ELISA 试剂盒

This 小鼠 Renin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Renin.
Rockland
产品编号 ABIN6719769
Supplier Product No.: koa0736
发货至: 中国

Quick Overview for Renin ELISA 试剂盒 (ABIN6719769)

抗原

See all Renin (REN) ELISA试剂盒
Renin (REN)

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0736

    Supplier

    Rockland

    原理

    Mouse Renin-1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Renin-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 62.5pg/mL-4000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Renin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Renin-1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: S72-R402

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Renin (REN) ELISA试剂盒

    Renin (REN)

    别名

    Renin-1

    背景

    Synonyms: ATP6AP2, ATP6M8-9, ATPase H(+)-transporting lysosomal accessory protein 2, ATPase H(+)-transporting lysosomal-interacting protein 2, Embryonic liver differentiation factor 10, ER-localized type I transmembrane adaptor, N14F, Renin receptor, Renin/prorenin receptor, RENR_HUMAN, V-ATPase M8.9 subunit, Vacuolar ATP synthase membrane sector-associated protein M8-9

    Background: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.

    基因ID

    19701

    NCBI登录号

    NP_112469

    UniProt

    P06281

    途径

    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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